![]() On the other hand, some antibodies will only recognize epitopes on proteins in their native, folded state. Many antibodies will only recognize proteins that have been reduced and denatured, because this reveals epitopes that would otherwise be obscured by secondary and tertiary folding of the proteins. Some antibodies require samples to be treated in a specific manner. For example, if you are trying to detect a cell surface protein on live cells by FACS, choose an antibody that is raised against an extracellular domain of the protein. The immunogen is generally described on the datasheet (in some cases an exact description of the immunogen is not given for proprietary reasons).Ĭheck that the immunogen is identical to or contained within the region of the protein you are trying to detect. Immunogens can be full-length proteins, protein fragments, peptides, whole organisms (for example, bacteria), or cells. The region of the protein that you wish to detectĪntibodies are generated by immunizing host animals with an immunogenic substance. The nature of your sample will determine which antibody is most appropriate. For assistance with your application, browse our protocol library. Out antibodies are continuously tested in-house and datasheets are updated with latest application information. If an application is not listed, it means that we have not tested it and it is unknown how the antibody will perform. When an antibody is tested in an application and fails, this is also noted on the datasheet. Antibody datasheets list the applications we have successfully tested the antibody in.
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